Vol 3 , Issue 1 , January - March 2015 | Pages: 330-336 | Research Paper
Received: January 04, 2015 | Revised: January 10, 2015 | Accepted: January 20, 2015 | Published Online: March 15, 2015
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Introduction: Methicillin resistant Staphylococcus aureus (MRSA) is endemic in India. In the present era of antibiotic resistance, the emergence of multi-drugs resistant organism is becoming more common. The present study is performed in patients admitted in hospital in various department of hospital to study the infection rate of MRSA from these units. Objectives: The aim of this study was to isolate Staphylococcus aureus from various samples of patients admitted in tertiary care hospital, & to study the prevalence rate of MRSA in the hospital. Material and method: The study was conducted in TMMC & RC hospital from January 2013 to Sepetember2013. A total of one hundred Staphylococcus aureus isolates from clinical samples were subjected to MRSA screening using Kirby-Bauer methods with cefoxitin 30μg discs per CLSI guidelines. Results: There were 225 different samples collected and all the samples were inoculated on Blood agar medium than incubated. After overnight incubation 100 Staphylococcus aureus were isolated, among which 26 were MRSA and 74 were MSSA has been isolated.The susceptibility pattern of antibiotics showed that all MRSA isolates were significantly less sensitivity to antibiotics as compared to MSSA. None of the MRSA strain showed resistance towards Vancomycin & Linezolid. Conclusion: The emergence of MRSA and other antibiotic resistant organisms reflects widespread ubiquitous over-use of antibiotics on a global scale. Control measures are required to prevent spread and reduce emergence of resistance.
Keywords
Methicillin Resistant Staphylococcus Aureus (MRSA); Cefoxitin; Blood Agar Medium; Vancomycin & Linezolid