Journal Press India®

Evaluation of Green Tea Effect on Oral Bacteria, Streptococcus Mutans

Vol 9 , Issue 1 , January - March 2021 | Pages: 15-24 | Research Paper  

https://doi.org/10.51976/ijari.912103

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Author Details ( * ) denotes Corresponding author

1. * Riya Kukreti, Department of Biotechnology, Khurana Block, Meerut Institute of Engineering and Technology, Meerut, Uttar Pradesh, India (kukretiriya@gmail.com)
2. Poonam Rani, Department of Biotechnology, Khurana Block, Meerut Institute of Engineering and Technology, Meerut, Uttar Pradesh, India
3. Rohit Gautam, Department of Biotechnology, Khurana Block, Meerut Institute of Engineering and Technology, Meerut, Uttar Pradesh, India

Tea is a gift of China to the world. It is a popular beverage consumed by almost every person globally, after water. Green tea has not gone under oxidation process and found beneficial for human consumption because of its medicinal properties and presence of some nutrients and minerals required by human beings for a healthy life. Molecular component of green tea (Polyphenols, catechins, flavonoids and minerals) makes it suitable in ailments and some microbial infections. Oral cavity has the second largest and diverse microbiota after gut, as numerous microorganisms such as bacteria, fungi and viruses exist in the mouth. Streptococcus mutans is facultative, anaerobic oral bacteria mainly responsible for tooth decay was selected for the present study because dental plaque and caries are common infections found in population. For experimental purpose 30 healthy individuals were randomly selected from the college campus (MIET, Meerut). Saliva of each individual was taken twice i.e. before and after green tea consumption. For identification and confirmation of S. mutans, biochemical tests have been done. The effect of green tea was observed by the growth and count of S. mutans on common (nutrient agar media) and special media (Mitis Salivary agar media). Plates were showing less count after green tea consumption as compared to the plates before consumption of green tea. On the basis of the results, it can be concluded that Green tea is the natural preventive and curative agents and is good to consume once in a day to reduce the count of Streptococcus mutans in mouth to maintain oral health.

Keywords

Green tea; Streptococcus mutans; Polyphenol; Medicinal Properties; Antibacterial


  1. JA Aas, BJ Paster, LN Stokes, I Olsen, FE Dewhirst. Defining the Normal Bacterial Flora of the Oral Cavity, Journal of clinical Microbiology, 43(11), 2005, 5721-5732.

  2. S Aryal. Nutrient agar – composition, preparation and uses, Microbiology Info.com, 2018.

  3. S Aryal. Gram staining, principle, reagent, procedure steps and result, Microbe notes, 2020. http://microbenotes.com/gram-stain-principle-reagent-procedure-and-result-interpretation/.

  4. JA Banas. Virulence properties of Streptococcus mutans, Frontiers in bioscience, a journal and virtual library, 9, 2004, 1267-1277.

  5. C Cabera, R Artacho, R Gimenez. Beneficial effects of green tea-A review, J Am Coll Nutr, 25, 2006, 79-99.

  6. A Choudhary. Working principle of pH meter, Pharmaceutical guidelines, 2015.

  7. A Connelly. The laboratory balances a practical guide, andyconnelly.wordpress.com, 2017.

  8. ME Davey, GA O'toole. Microbial biofilms: from ecology to molecular genetics, Microbiology and Molecular Biology Reviews, 64, 2000, 847-867.

  9. FE Dewhirst, T Chen, J Izard, BJ Paster, ACR Tanner, WH Yu, A Lakshmanan, WG Wade. The human oral microbiome, Journal of Bacteriology, American Society for Microbiology, 2010.

  10. TM Elattar, AS Virji. Effect of tea polyphenols on growth of oral squamous carcinoma cells in vitro, Anticancer Res. 20, 2000, 3459–3465.

  11. M Elvin-Lewis, R Steelman. The anticariogenic effects of tea drinking among Dallas school children, J. Dent. Res, 65, 1986, 198.

  12. GF Ferrazzano, I Amato, A Ingenito, ADe Natale. Anticariogenic effects of polyphenols from plant stimulant beverages (cocoa, coffee, tea), Fitoterapia, 80, 2009, 255-262.

  13. SD Forssten, M Bjorklund, C Ovwehand. Streptococcus mutans, caries and stimulation models, Nutrients, 2010, 1-10.

  14. M Friedman. Overview of antibacterial, antitoxin, antiviral, and antifungal activities of tea flavonoids and teas, Molecular Nutrition and Food Research, 51, 2007, 16-34.

  15. Garg M, Top 4 tests for antimicrobial drug susceptibility, Biology Discussion, http://www.biologydiscussion.com/media-microbiology/top-4-tests-for-antimicribial-drug-susceptibility.

  16. JMT Hamilton-Miller. Anti-cariogenic properties of tea (Camellia sinensis), Journal of Medical Microbiology, 50(4), 2001, 299–302.

  17. Hilal. Morphology, Manufacturing, Types, Composition and Medicinal Properties of Tea (Camellia sinensis), Journal of Basic and Applied Plant Sciences, 1, 2017.

  18. K Hirao, H Yumoto, T Nakanishi, K Mukai, K Takahashi, D Takegawa. Tea catechins reduce inflammatory reactions via mitogen-activated protein kinase pathways in toll-like receptor 2 ligand-stimulated dental pulp cells, Life Sci, 86, 2010, 654-60.

  19. M Hirasawa, K Takada. Multiple effects of green tea catechin on the antifungal activity of antimycotics against Candida albicans, Journal of Antimicrobial Chemotherapy, 53, 2004, 225-9.

  20.  M Hirasawa, K Takada, S Otake. Inhibition of acid production in dental plaque bacteria by green tea catechins. Caries Res; 40, 2006, 265-70. 

  21. J Holt,  N Krieg, P Sneath,  J Staley,  S Williams, Bergey's manual of determinative bacteriology, 9th ed; Williams & Wilkins: Baltimore, MA, USA1994.

  22. Hot air oven – principle, working mechanism, sterilization control, advantage and disadvantage, http://universe84a.com/hot-air-oven.

  23. Z Khamverdi, M Azarsina. The Beneficial Effects of Green Tea in Oral Health and Dentistry, Biomedical journal of scientific and technical research, 2019

  24. M Kilian. The oral microbiome – friend or foe, European Journal of Oral Science, 126, 2018, S1.

  25. P Koll, R Mandar, H Marcotte, E Leibur, M Mikelsaar, L Hammarstrom. Characterization of oral lactobacilli as potential probiotics for oral health, Oral Microbiology and Immunology, 23, 2008, 139-147.

  26. P Kumari. Effects of green ten in oral health, International journal of scientific engineering and research, 6, 2018.

  27. RJ Lamont, DR Demuth, CA Davis, D Malamud, B Rosan.  Salivary-Agglutinin-Mediated Adherence of Streptococcus-Mutans to Early Plaque Bacteria, Infection and Immunity, American society for microbiology, 59, 1991, 3446-3450.

  28. JA Lemon, RG Quivey, H Koo, J Abranches. Streptococcus mutans: a new gram-positive paradigm, Microbiology, 159.

  29. N Li, C Han, J Chen. Tea preparations protect against DMBA induced oral carcinogenesis in hamsters, Nutrition, Cancer, 35, 1999°, 73–79.

  30. N Li, Z Sun, C Han, J Chen. The chemopreventive effects of tea on human oral precancerous mucosa lesions, Proceedings of the Society for Experimental Biology and Medicine/, 220 (4), 1999b, 218–224.

  31. HA Linke, RZ LeGeros. Black tea extract and dental caries formation in hamsters. Int J Food Sci Nutr, 54, 2003, 89-95.

  32. P Lodhia, K Yaegaki, A Khakbaznejad, T Imai, T Sato, T Tanaka.  Effect of green tea on volatile sulfur compounds in mouth air, Journal of Nutritional Science and Vitaminology, 54, 2008, 89-94.

  33. WJ Loesche. Role of Streptococcus mutans in human dental decay. American society of microbiology, Microbiological review, 50, 1986, 353-380.

  34. Lu M, Wang Z and Xuan S (2019), Oral Microbiota: A new view of body health, Food Science and Human Wellness, ScienceDirect, 8.

  35. T Mahmood, N Akhtar, BA Khan. The morphology, characteristics, and medicinal properties of Camellia sinensis’ tea, Journal of Medicinal Plants Research, 4(19), 2010.

  36. L Mao-Jung, DL Joshua, P Saileta, M Xiaofeng. Delivery of Tea Polyphenols to the Oral Cavity by Green Tea Leaves and Black Tea Extract. Cancer Epidemiol. Biomarkers Prev. 13, 2004, 132–137.

  37. P Marsh. Dental plaque as a biofilm and a microbial community - implications for health and disease. BMC Oral Health, 6, 2006, S14.

  38. KH Metwalli, SA Khan, MA Jabra-Rizk. Streptococcus mutans, Candida albicans, and the Human Mouth: A stcky Situation, Plos Pathogens 2013.

  39. M Moezizadeh. Anticariogenic effect of tea: A review of literature, Journal of Dentistry and Oral Hygiene, 5(9), 2013.

  40. A Moghbel, A Farjzadeh,  N Aghel, H Agheli, N Raisi. The Effect of Green Tea on Prevention of Mouth Bacterial Infection, Halitosis, and Plaque Formation on Teeth, Iranian Journal of Toxicology, 5, 2011.

  41. AnchorM Mohan, G Jeevanandan, SM Raja. The role of green tea in oral health - A Review, Asian journal of pharmaceutical and clinical research, (11), 2018, 2-3.

  42. M Onisi, F Ozaki, F Yoshino, Y Murakami. An experimental evidence of caries preventive activity of non-fluoride component in tea. Koku Eisei Gakkai Zasshi, 31(2), 1981b, 158–162.

  43. Onisi M, Shimura N, Nakamura C and Sato M (), A field test on the caries preventive effect of tea drinking. J. Dent. Health 31(1) 1981, 13–19.

  44. T Ooshima, T Minami, W Aono, Y Tamura, S Hamada. Reduction of dental plaque deposition in humans by oolong tea extract. Caries Res, 28, 1994, 146–149.

  45. S Otake, M Makimura, T Kuroki, Y Nishihara, M Hirasawa . Anticaries effects of polyphenolic compounds from Japanese green tea. Caries Res, 25, 1991, 438-443.

  46. MA Pfaller, DJ Diekema, RN Jones, SA Messer, RJ Hollis. Sentry Participants Group, Trends in antifungal susceptibility of Candida spp. Isolated from pediatric and adult patients with bloodstream infections: SENTRY antimicrobial surveillance program, 1997 to 2000, Journal of Clinical Microbiology, 40, 2002, 852-856.

  47. Ramesh K, Rajesh E, Devi GN and Jimson S (2016), Green tea in oral health - A Review, Biomedical & Pharmacology Journal, Vol. 9(2), 2016, 851-852.

  48. Ramesh, E Rajesh, GN Devi, S Jimson. Green Tea in Oral Health – A Review, Biomedical and Pharmacology Journal, 2016. 

  49. U Ranganathan, CH Akhila. Streptococcus mutans: has it become prime perperator for oral manifestations, Journal of Microbiology and eaperimentation, 2019.

  50. S Rassameemasmaung, P Phusudsawang, V Sangalungkarn. Effect of green tea mouthwash on oral malodor. ISRN 2013, 975148.

  51. M Sakamoto, M Umeda, Y Benno. Molecular analysis of human oral microbiota. J. Periodont. Res, 40, 2005, 277-285. 

  52. S Sakanaka, T Sato, M Kim, T Yamamoto. Inhibitory Effects of Green Tea Polyphenols on Glucan Synthesis and Cellular Adherence of Cariogenic Streptococci, Agricultural and Biological. Chemistry, 54 (11), 1990, 2925-2929.

  53. A Sapkota. Autoclave – definition, parts, principle, procedure, types and uses, Microbe notes, 2020.

  54. A Sapkota. Instruments used in microbiology lab with principle and uses, microbe notes, 2020.

  55. A Sapkota. Laminar flow hood/cabinet – definition, parts, principle, uses, types, Microbe notes, 2020.

  56. A Sapkota. Nutrient agar-principle, composition, preparation, results, uses, Microbe notes, 2020.

  57. S Sarkar, A Chowdhury, S Das, B Chakraborty, P Mandal, M Chowdhury. Major tea processing particles in India, Original research article, International Journal of Bioassays, 2016.

  58. S Sehgal. Dental Caries are formed, 2019. https://medium.com/radiantstardental/how-dental-caries-are-formed.

  59. MG Shepherd. The pathogenesis and host defence mechanisms of oral candidosis, N Z Dent J, 82, 1986, 78-81.

  60. V Singh, Kuldeep, A Bajaj, M Kalra. Green tea: enhancing oral health, Journal of   Dental Sciences University, 1, 2016.

  61. JM  Song, KH Lee, BLSeong. Antiviral effect of catechins in green tea on influenza virus, Antiviral Res, 68, 2005, 66-74.

  62. S Soni, P Jain, VK Chaudhary. Beneficial effects of polyphenolic beverage on oral and Periodontal health, Journal of contemporary denta sciences, 1 (1)  33-41.

  63. A Staskova, R Nemcova, S Lauko, A Jenca. Oral Microbiota from the Stomatology Perpective, Open access peer-reviewed chapter, 2019.

  64. JM Tanzer, J Livingston, AM Thompson. The microbiology of primary dental caries in humans, J. Dent. Educ, 65, 2001, 1028-1037.

  65. Trisha, Microscopy and properties of microscope, Biology discussion,http://www.biologydiscussion.com/microscope/micoscopy-&-properties-of- microscope.

  66. WG Wade. The oral microbiome in health and disease, Pharmacological research, ScienceDirect, 69(1), 2013, 137-143.

  67. JH Weisburger, A Rivenson, Caliaga. Effect of tea extracts, polyphenols, and epigallocatechin gallate on azoxymethane-induced colon cancer, proceedings of the society for experimental biology and medicine, 217, 1998, 104-108.

  68. CD Wu, GX Wei. Tea as a functional food for oral health, Nutrition, 18, 2002, 443-444.

  69. M Yoshiharu, K Kazuko, H Yukio, T Toshio. Anti-demineralizing potential of bottled sugar-free green tea beverages in vitro, Oral Sci. Int, 6, 2009, 21-26.

  70.  H Yu, T Oho, S Tagomori, T Morioka, Anticariogenic effects of green tea, Fukuoka Igaku Zasshi, 83, 1992, 174-180.

  71. Zelnicek T (2016), Streptococcus mutans – Tooth Decay, Microwiki,http://microbewiki. kenyon.edu/index.php/streptococcus-mutans-Tooth-Decay.

  72. J Zhang, S Kashket. Inhibition of salivary amylase by black and green teas and their effects on the intraoral hydrolysis of starch. Caries Research, 32, 1998, 233-238.

  73. AnchorM Mohan, G Jeevanandan, SM Raja. The role of green tea in oral health - A Review, Asian journal of pharmaceutical and clinical research, (11), 2018, 2-3.


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